ISOLATION AND CHARACTERIZATION OF A PLASTIC DEGRADING MICROBE FROM INSECT GUT

Main Article Content

Swathi Lakshmi
Vinita Balasubramanya

Abstract

Microbial degradation of plastics has been traditionally studied in soil microbes. In this study, plastic-degrading microbes were isolated from the midgut and hindgut of cockroach (Periplaneta americana) using minimal media containing polyethylene glycol (PEG) and polyvinyl pyrrolidone (PVP). These microbes could also survive in polyethylene and polyvinyl chloride (PVC) containing media, as confirmed by decrease in weight, thin layer chromatography and FTIR analysis. Identified as strains of Enterobacter cloacae and Klebsiella pneumoniae, these microbes show promising potential for sustainable plastic biodegradation and mitigation of plastic pollution.

Article Details

Section

Articles

Author Biography

Swathi Lakshmi

Department of PG Biotechnology, NMKRV College, Bengaluru – 560011

How to Cite

ISOLATION AND CHARACTERIZATION OF A PLASTIC DEGRADING MICROBE FROM INSECT GUT. (2026). International Journal of Fundamental and Applied Sciences (IJFAS), 15(1), 53-70. https://doi.org/10.59415/ijfas.341

References

Ahmed, T., Shahid, M., Azeem, F., Rasul, I., Shah, A. A., Noman, M., Hameed, A., Manzoor, N., Manzoor, I., & Muhammad, S. (2018). Biodegradation of plastics: current scenario and future prospects for environmental safety. Environmental science and pollution research international, 25(8), 7287–7298. https://doi.org/10.1007/s11356-018-1234-9 DOI: https://doi.org/10.1007/s11356-018-1234-9

Awasthi, S., Srivastava, P., Singh, P., Tiwary, D., & Mishra, P. K. (2017). Biodegradation of thermally treated high-density polyethylene (HDPE) by Klebsiella pneumoniae CH001. 3 Biotech, 7(5), 332. https://doi.org/10.1007/s13205-017-0959-3 DOI: https://doi.org/10.1007/s13205-017-0959-3

Bertino-Grimaldi, D., Medeiros, M. N., Vieira, R. P., Cardoso, A. M., Turque, A. S., Silveira, C. B., Albano, R. M., Bressan-Nascimento, S., Garcia, E. S., de Souza, W., Martins, O. B., & Machado, E. A. (2013). Bacterial community composition shifts in the gut of Periplaneta americana fed on different lignocellulosic materials. SpringerPlus, 2, 609. https://doi.org/10.1186/2193-1801-2-609 DOI: https://doi.org/10.1186/2193-1801-2-609

Cao, Z., Yan, W., Ding, M. & Yuan, Y. (2022). Construction of microbial consortia for microbial degradation of complex compounds. Frontiers in Bioengineering and Biotechnology 10: https://doi.org/10.3389/fbioe.2022.1051233 DOI: https://doi.org/10.3389/fbioe.2022.1051233

Cheng, F., Zhang, T., Liu, Y., Zhang, Y., & Qu, J. (2021). Non-negligible effects of UV irradiation on transformation and environmental risks of microplastics in the water environment. Journal of xenobiotics, 12(1), 1–12. https://doi.org/10.3390/jox12010001 DOI: https://doi.org/10.3390/jox12010001

Das, M.P., & Kumar, S. (2015). An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech 5, 81–86 https://doi.org/10.1007/s13205-014-0205-1 DOI: https://doi.org/10.1007/s13205-014-0205-1

Elahi, Z., A., Bukhari, D., A., Shamim, S., & Rehman, A. (2021). Plastics degradation by microbes: A sustainable approach. Journal of King Saud University - Science 33(6), 101538 https://doi.org/10.1016/j.jksus.2021.101538 DOI: https://doi.org/10.1016/j.jksus.2021.101538

Ganesh Kumar, A., Anjana, K., Hinduja, M., Sujitha, K. & Dharani, G. (2020). Review on plastic wastes in marine environment–Biodegradation and biotechnological solutions. Marine Pollution Bulletin, 150, 110733. https://doi.org/10.1016/j.marpolbul.2019.110733 DOI: https://doi.org/10.1016/j.marpolbul.2019.110733

Hu, J., Peng, B., Liu, Z., Long, T., Gao, Wa., Wang, Y., Liu, X. & Zhao, J. (2025). Polyethylene degradation mediated by Klebsiella variicola isolated from the gut of insect larvae. International Biodeterioration & Biodegradation 205: 106182 https://doi.org/10.1016/j.ibiod.2025.106182 DOI: https://doi.org/10.1016/j.ibiod.2025.106182

Jacquin, J., Cheng, J., Odobel, C., Pandin, C., Conan, P., Pujo-Pay, M., Barbe, V., Meistertzheim, A. L., & Ghiglione, J. F. (2019). Microbial ecotoxicology of marine plastic debris: A review on colonization and biodegradation by the “Plastisphere”. Frontiers in Microbiology, 10, 865. https://doi.org/10.3389/fmicb.2019.00865 DOI: https://doi.org/10.3389/fmicb.2019.00865

Khurana, S., Ali, S., Srivastava, A., K., Singh, A., Agarwal, H., Chauhan, R., Joshi, N., C., Dufoss, L., & Chauhan, A. (2025). Bioremediation of microplastic pollution: A systematic review on mechanism, analytical methods, innovations, and omics approaches. Journal of Hazardous Materials Advances 19: 100777 https://doi.org/10.1016/j.hazadv.2025.100777 DOI: https://doi.org/10.1016/j.hazadv.2025.100777

Mohamed, S. F., & Narayanan, R. (2024). Enterobacter cloacae-mediated polymer biodegradation: in-silico analysis predicts broad spectrum degradation potential by Alkane monooxygenase. Biodegradation, 35(6), 969–991. https://doi.org/10.1007/s10532-024-10091-4 DOI: https://doi.org/10.1007/s10532-024-10091-4

Plastics Europe. Plastics-The Facts 2020: An analysis of European plastics production, demand and waste data. Retrieved from https://www.plasticseurope.org/application/files/8016/1125/2189/AF_Plastics_the_facts-WEB-2020-ING_FINAL.pdf (accessed on 23 March 2025).

Sarker, R. K., Chakraborty, P., Paul, P., Chatterjee, A., & Tribedi, P. (2020). Degradation of low-density poly ethylene (LDPE) by Enterobacter cloacae AKS7: a potential step towards sustainable environmental remediation. Archives of microbiology, 202(8), 2117–2125. https://doi.org/10.1007/s00203-020-01926-8 DOI: https://doi.org/10.1007/s00203-020-01926-8

Sen, S., K., & Raut, S. (2015). Microbial degradation of low-density polyethylene (LDPE): A review. Journal of Environmental Chemical Engineering 3(1), 462–473. https://doi.org/10.1016/j.jece.2015.01.003 DOI: https://doi.org/10.1016/j.jece.2015.01.003

Silva, R. R. A., Marques, C. S., Arruda, T. R., Teixeira, S. C., & de Oliveira, T. V. (2023). Biodegradation of polymers: Stages, measurement, standards and prospects. Macromolecules, 3(2), 371-399. https://doi.org/10.3390/macromol3020023 DOI: https://doi.org/10.3390/macromol3020023

Sumathi, T., Viswanath, B., Lakshmi, A.S., & Sai Gopal, D.V.R. (2016). Production of laccase by Cochliobolus sp. isolated from plastic dumped soils and their ability to degrade low molecular weight PVC. Biochemistry research international, 2016, 9519527. https://doi.org/10.1155/2016/9519527 DOI: https://doi.org/10.1155/2016/9519527

Tokiwa, Y., Calabia, B. P., Ugwu, C. U., & Aiba, S. (2009). Biodegradability of plastics. International journal of molecular sciences, 10(9), 3722–3742. https://doi.org/10.3390/ijms10093722 DOI: https://doi.org/10.3390/ijms10093722

Zhang, X., Feng, X., Lin, Y., Gou, H., Zhang, Y., & Yang, L. (2023). Degradation of polyethylene by Klebsiella pneumoniae Mk-1 isolated from soil. Ecotoxicology and environmental safety, 258, 114965. https://doi.org/10.1016/j.ecoenv.2023.114965 DOI: https://doi.org/10.1016/j.ecoenv.2023.114965

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