https://journals.mlacwresearch.org/index.php/ijfas/issue/feedInternational Journal of Fundamental and Applied Sciences (IJFAS)2026-02-05T11:37:10+00:00Dr Sushil Kumar Middhaijfas@mlacw.edu.inOpen Journal SystemsIJFAS (ISSN: 2278-1404) is a peer-reviewed open-access journal publishing original research in fundamental and applied biological sciences.https://journals.mlacwresearch.org/index.php/ijfas/article/view/314IDENTIFICATION AND SPECIATION OF DIFFERENT SPECIES OF KLEBSIELLA ISOLATED FROM VARIOUS CLINICAL SAMPLES AND THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL2026-02-05T11:37:10+00:00Areeba Rehmansupport@mypadnow.comShadma Yaqoobsupport@mypadnow.comNighat Parveennighatparveenkhan46@gmail.comSaumya Shuklasupport@mypadnow.comSanjay Kumar Vishwakarmasanjayrise@gmail.com<p><em>Klebsiella </em>is a member of the fifth of the eight tribes of the Enterobacteriales family. The second most prevalent organism in the Enterobacteriaceae family is <em>Klebsiella</em> Species. It is a rod-shaped, facultative anaerobic bacillus that ferments lactose and is gram negative. Both hospital-acquired infections & community-acquired infections are known to be frequently caused by them. Additionally, <em>Klebsiella</em> is the second most frequent cause of septicemia and bacteremia at hospital paediatric wards, particularly on intensive care units and premature infants. <em>Klebsiella</em> species are the causative agents of urinary tract infections, pneumonia, abrasion, muscle & bacteremia and account for 3-8% of all nosocomial infections. To identify <em>Klebsiella</em> Species prevalence in different clinical samples along with to evaluate its antibiotic sensitivity method isolated from variety of clinical samples. Over the course of the six- month trial period, 74 samples in total were analyzed. To identify and isolate <em>Klebsiella</em> Species, each of them underwent direct microscopy and culture. In this study 42(57%) <em>Klebsiella</em> Species were isolated in female patients and 32(43%) were from male patients which is identified out of 74 clinical samples. Among the isolates, <em>K. pneumoniae </em>was identified in 65 cases (87.84%) and <em>K. oŃ…ytoca</em> in 9 cases (12.16%). Urine and Blood samples where the majority of <em>Klebsiella</em> Species were isolated from specimens. Higher sensitivity was seen with Colistin (100%), Tigecycline (94.59%) and Doxycycline (83.78%) among the 30 urine isolates tested, (83.33%) were sensitive to Fosfomycin by Kirbey-Bauer disc diffusion method. High resistance rates were recorded against: Ceftriaxone (87.84%) and Amoxicillin (79.73%). As an organism with a high prevalence that is growing more common due to antimicrobial agent resistance, <em>Klebsiella </em>Species may pose a risk to health.</p> <p> </p>2025-12-30T00:00:00+00:00Copyright (c) 2026 Areeba Rehman, Shadma Yaqoob, Nighat Parveen, Saumya Shukla, Sanjay Kumar Vishwakarma