PHYTOCHEMICAL CONSTITUENTS AND BIOACTIVE POTENTIAL OF ACHILLEA LANULOSA EXTRACT

2026-03-30T04:55:29+00:00

Achillea is a genus known for its potent medicinal properties and, hence plants of this genus have been used traditionally for the treatment of fever, pain, wounds healing and inflammation in different parts of the world. Considering the use of this plant and its products at such large scale in treating different ailments, the scientific validation of such claims becomes very important. A. lanulosa is one of the least studied varieties from Achillea genus that might have great medicinal importance. Therefore, the current study has been undertaken to explore the phytochemical constituents, antioxidant and antibacterial properties of A. lanulosa extract prepared in 80% methanol. Phytochemical profiling of the extract by qualitative and quantitative assays has revealed the presence of phytoconstituents including phenolics and flavonoids. Gas chromatography and Mass spectrometry of the extract showed significant concentration of 20 compounds including polyhydroxy acids, flavonoids and tocopherols and different type of terpenes. The DPPH scavenging assay had shown the significant antioxidant potential of the extract with IC₅₀ of 24.5 ± 3.56 µg/ml. Further, the well diffusion assay demonstrated a pronouncedantibacterial activity in the plant extract against Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pyogenes, and Enterobacter sp. The findings of this study highlight the therapeutic potential of A. lanulosa, which may be helpful and promising option for additional research to fight bacterial infections and enhance human health.

ISOLATION AND CHARACTERIZATION OF ANTIMICROBIAL BACTERIAL ENDOPHYTES AGAINST FOODBORNE PATHOGENS FROM COFFEA ARABICA & COFFEA ROBUSTA FROM CHIKMAGALUR

2026-03-30T04:55:26+00:00

Endophytes are microorganisms that reside within plant tissues without causing any harm to the host. These non-pathogenic symbionts are known to produce various bioactive compounds, including antibiotics. Recent studies have emphasized the potential of endophytic bacteria in addressing the escalating threat of antibiotic resistance among pathogenic microorganisms. Chikmagalur, widely recognized as the birthplace of Indian coffee and known as the "Coffee Land of Karnataka," is renowned for the cultivation of Coffea arabica and Coffea robusta. In the present study sixteen bacterial endophytic isolates were obtained from different parts including root, stem, leaves and bark of Coffea arabica & Coffea robusta from Chikmagalur. The antimicrobial screening of the ethyl acetate extract of the isolates against foodborne pathogens, such as Salmonella typhi, Shigella, E. coli & Enterococcus faecalis was done using agar diffusion method. Eight out of sixteen endophytic bacterial isolates exhibited antimicrobial activity against the tested foodborne pathogens. Among them, two isolates CCRBI and CCRB2, both obtained from the bark of Coffea robusta demonstrated notably higher antimicrobial activity, showing larger zones of inhibition against Salmonella typhi, Shigella spp., and Escherichia coli. Consequently, these two potent isolates were selected for detailed morphological, biochemical, and phytochemical characterization.

Bioactive compounds were extracted from the liquid cultures of CCRBI and CCRB2 using ethyl acetate, and the resulting crude extracts were subjected to phytochemical analysis to identify the secondary metabolites. The ethyl acetate extract of CCRBI exhibited inhibition zones of 20.5 mm against S. typhi, 26.5 mm against Shigella, and 24 mm against E. coli. Similarly, CCRB2 showed inhibition zones of 20.5 mm, 21.5 mm, and 27 mm against the same pathogens, respectively. Phytochemical screening revealed the presence of alkaloids and saponins in the crude extracts, indicating their potential role in the observed antimicrobial activity.

International Journal of Fundamental and Applied Sciences (IJFAS)

PHYTOCHEMICAL CONSTITUENTS AND BIOACTIVE POTENTIAL OF ACHILLEA LANULOSA EXTRACT

ISOLATION AND CHARACTERIZATION OF ANTIMICROBIAL BACTERIAL ENDOPHYTES AGAINST FOODBORNE PATHOGENS FROM COFFEA ARABICA & COFFEA ROBUSTA FROM CHIKMAGALUR