In vitro Antioxidant and free radical scavenging activity of Macrotyloma uniflorum dal from Kumauni region
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Abstract
The present study was carried out to evaluate the in vitro antioxidant activities of Methanol
extract of Dolichos biflorus dal ((DME)) commonly edible food from central Himalayans. Methods: This was
achieved by screening of the plant extracts at varying concentrations (20-200μg/ml), using 2,2-diphenyl-1-picryl-
hydrazyl (DPPH) radical scavenging activity, reducing power assay and hydrogen peroxide radical scavenging activity.
Results: Total phenol and flavonoid contents (92.10 ± 8.11 mg/ml GAE per 100 mg plant extract and 139.5 ± 55.09
mg/ml QE equivalent per 100 mg plant extract) were found respectively. Scavenging effect of DME was 4 times greater
than that of the synthetic antioxidant ascorbic acid. Conclusion: Result also suggests a close relations in between total
phenolic content and antioxidant activity, reducing power and radical scavenging effect on DPPH radicals, which proves
Dolichos biflorus has a potential source of useful natural antioxidants.
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