CORRELATION BETWEEN VITAMIN B12 DEFICIENCY ANEMIA AND ARGININE METHYLATION IN ERYTHROCYTE BAND 3
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Abstract
Vitamin B12 deficiency is marked by megaloblastic anemia and abnormal red blood cell morphology. This study attempts to correlate B12 levels with RBC membrane proteins (especially Band 3) and arginine methylation. B12-deficient, iron-deficient and normal blood samples were analysed using SDS-PAGE and in silico using Alphafold3. SDS-PAGE revealed differences in band intensity and size, indicating altered membrane protein profiles in B12-deficient samples. AlphaFold3 modelling of known Band 3 mutations involving arginine substitutions revealed structural changes, especially in the N-terminal domain responsible for interaction with other membrane proteins. These preliminary observations suggest a possible correlation between B12 deficiency, arginine methylation, and changes in RBC membrane structure.
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