Effect of controlled Nickel exposure on Duttaphrynus melanostictus oxidative stress and its management

Abstract

This study mainly focuses on the effect of a common heavy metal poisoning and how the oxidative stress is managed in Duttaphrynus melanostictus during Nickel poisoning. The subject, Duttaphrynus melanostictus was exposed to Nickel in regular frequency with a very small amount by creating moist environment which imitates the presence of Nickel in soil due to pollution and then the body weight analysis (by ANOVA and Post Hoc analysis (using SPSS package 16.0)) and skin coloration and pigmentation were checked at 24 hours interval to study the oxidative stress and its management by the animal's physiological system. The initial change in skin color at early stages of nickel exposure and then its restoration was observed. Similarly the loss of body weight at initial exposure and then its stabilization after some days was noted. The analysis and observations revealed that the antioxidant system of Duttaphrynus melanostictus is well developed to sustain against the oxidative stress due to nickel exposure.