IMPACT OF FERMENTATION TIME ON NUTRITIONAL AND FUNCTIONAL QUALITY OF PROBIOTIC CURD

Main Article Content

Nuthana Jain V
Monisha T
Shruti Awasthi
Preethi Rajesh
Chethana V Chalapathy

Abstract

Fermented dairy products are a good source for probiotics with health benefits that are aligned with sustainable development goals. This study understands the effect of fermentation time on the nutritional constitutes and functional quality of commercial probiotic curd. Inoculum curd was collected from retail outlets and was incubated at 37°C and analyzed at 4 different fermentation time intervals: 0, 6, 12 and 24 hours. Probiotic viability was evaluated by standard plate count (SPC) method on MRS agar and phytochemical parameters - pH, titratable acidity, syneresis were analysed using standard methods. Nutritional analysis - reducing sugar and proteins were determined by standard techniques like titration and colorimetric techniques. This analysis is mainly focused to identify the best fermentation time for a probiotic survival which contains desired nutritional and functional qualities. By observing the microbial growth pattern, acidity, nutrient components over time helped us to understand the optimal fermentation time required to maintain both the product quality and potential health benefits. These insights are intended to guide the producers in designing and optimizing the fermentation protocol to enhance consumer health while also focusing on keeping consumers informed about the voice of probiotic dairy consumption. This research highlights how science-driven optimization of traditional dairy processing can contribute to local food sustainability, consumer health, and broader public health goals.


 

Downloads

Download data is not yet available.

Article Details

How to Cite
Nuthana Jain V, Monisha T, Shruti Awasthi, Preethi Rajesh, & Chethana V Chalapathy. (2026). IMPACT OF FERMENTATION TIME ON NUTRITIONAL AND FUNCTIONAL QUALITY OF PROBIOTIC CURD. International Journal of Fundamental and Applied Sciences (IJFAS), 15(1), 25–36. https://doi.org/10.59415/ijfas.334
Section
Articles
Author Biographies

Nuthana Jain V, Garden City University,Bengaluru, Karnataka, India

PG Student, Department of Life Sciences, Garden City University, Bengaluru

Monisha T, Garden City University,Bengaluru, Karnataka, India

PG Student, Department of Life Sciences, Garden City University, Bengaluru

Shruti Awasthi, Garden City University,Bengaluru, Karnataka, India

Professor, Department of Life Sciences, Garden City University, Bengaluru

Preethi Rajesh, Garden City University,Bengaluru, Karnataka, India

Professor and Head, Department of Life Sciences, Garden City University, Bengaluru

Chethana V Chalapathy, Garden City University,Bengaluru, Karnataka, India

Professor, Department of Life Sciences, Garden City University, Bengaluru

References

Merenstein DJ, Tancredi DJ, Karl JP, Krist AH, Lenoir-Wijnkoop I, Reid G, et al. Is there evidence to support probiotic use for healthy people? Adv Nutr. 2024;15(8):100265. https://doi.org/10.1016/j.advnut.2024.100265 DOI: https://doi.org/10.1016/j.advnut.2024.100265

Terpou A, Papadaki A, Lappa IK, Kachrimanidou V, Bosnea LA, Kopsahelis N. Probiotics in food systems: significance and emerging strategies towards improved viability and delivery. Nutrients. 2019;11(7):1591. https://doi.org/10.3390/nu11071591 DOI: https://doi.org/10.3390/nu11071591

Byakika S, Mukisa IM, Byaruhanga YB, Muyanja C. A review of criteria and methods for evaluating probiotic potential of microorganisms. Food Rev Int. 2019;35(5):427–466. https://doi.org/10.1080/87559129.2019.1584815 DOI: https://doi.org/10.1080/87559129.2019.1584815

Periyatt Veettil S, Presanna Kumar S, Venkatesh T. Characterization and molecular identification of probiotic bacteria and proliferation analysis. Food Biosci. 2025;69:106809. https://doi.org/10.1016/j.fbio.2025.106809 DOI: https://doi.org/10.1016/j.fbio.2025.106809

Kumar R, Sood U, Gupta V, et al. Recent advancements in development of modern probiotics. Indian J Microbiol. 2020;60:12–25. https://doi.org/10.1007/s12088-019-00808-y DOI: https://doi.org/10.1007/s12088-019-00808-y

Kumari M, Patel HK, Kokkiligadda A, Bhushan B, Tomar SK. Development of fermented soymilk with improved properties. LWT. 2022;154:112827. https://doi.org/10.1016/j.lwt.2021.112827 DOI: https://doi.org/10.1016/j.lwt.2021.112827

Wilkinson MG. Flow cytometry for measuring probiotic viability: a review. Trends Food Sci Technol. 2018;78:1–10. https://doi.org/10.1016/j.tifs.2018.05.006 DOI: https://doi.org/10.1016/j.tifs.2018.05.006

Meng C, Bai C, Brown TD, Hood LE, Tian Q. Human gut microbiota and gastrointestinal cancer. Genomics Proteomics Bioinformatics. 2018;16(1):33–49. https://doi.org/10.1016/j.gpb.2017.06.002 DOI: https://doi.org/10.1016/j.gpb.2017.06.002

Neffe-Skocińska K, Rzepkowska A, Szydłowska A, Kołożyn-Krajewska D. Trends in probiotic use in food production. In: Holban AM, Grumezescu AM, editors. Handbook of Food Bioengineering. Academic Press; 2018. p. 65–94. DOI: https://doi.org/10.1016/B978-0-12-811446-9.00003-4

Knorr D. Technology aspects related to microorganisms in functional foods. Trends Food Sci Technol. 1998;9(8–9):295–306. https://doi.org/10.1016/S0924-2244(98)00051-X DOI: https://doi.org/10.1016/S0924-2244(98)00051-X

Hansen LT, Allan-Wojtas PM, Jin YL, Paulson AT. Survival of microencapsulated probiotics. Food Microbiol. 2002;19(1):35–45. https://doi.org/10.1006/fmic.2001.0452 DOI: https://doi.org/10.1006/fmic.2001.0452

Clark PA, Cotton LN, Martin JH. Selection of bifidobacteria for dietary use. Cult Dairy Prod J. 1993;28(4):11–14.

Akan E. Effect of fermentation time on probiotic goat yogurts. An Acad Bras Cienc. 2022;94(3):e20210875. DOI: https://doi.org/10.1590/0001-3765202220210875

Gomes AMP, Malcata FX. Probiotic bacteria properties. Trends Food Sci Technol. 1999;10(4–5):139–157. https://doi.org/10.1016/S0924-2244(99)00033-3 DOI: https://doi.org/10.1016/S0924-2244(99)00033-3

Dave RI, Shah NP. Viability of probiotic bacteria in yoghurt. Int Dairy J. 1997;7(1):31–41. https://doi.org/10.1016/S0958-6946(97)00017-6 DOI: https://doi.org/10.1016/S0958-6946(96)00046-5

Saarela M, Mogensen G, Fondén R, Mättö J, Mattila-Sandholm T. Probiotic microbes: from markets to mechanisms. Curr Opin Biotechnol. 2000;11(5):483–487.

Charteris WP, Kelly PM, Morelli L, Collins JK. Development of methodology for probiotic tolerance. J Appl Microbiol. 1998;84(5):759–768. DOI: https://doi.org/10.1046/j.1365-2672.1998.00407.x

Peng Y, Horne DS, Lucey JA. Impact of fermentation time on yogurt properties. J Dairy Sci. 2009;92(7):2977–2990. https://doi.org/10.3168/jds.2008-1221 DOI: https://doi.org/10.3168/jds.2008-1221

Ranadheera CS, Baines SK, Adams MC. Importance of food in probiotic efficacy. Food Res Int. 2012;48(2):946–954.

Shori AB. Influence of food matrix on probiotic viability. Food Biosci. 2016;13:1–8. https://doi.org/10.1016/j.fbio.2015.11.001 DOI: https://doi.org/10.1016/j.fbio.2015.11.001

Talwalkar A, Kailasapathy K. Role of oxygen in probiotic viability. Curr Issues Intest Microbiol. 2004;5(1):1–8.

Shah NP. Probiotic bacteria in dairy foods. J Dairy Sci. 2000;83(4):894–907. DOI: https://doi.org/10.3168/jds.S0022-0302(00)74953-8

Kailasapathy K. Survival of probiotic bacteria and sensory effects. LWT Food Sci Technol. 2006;39(10):1221–1227. DOI: https://doi.org/10.1016/j.lwt.2005.07.013

Vinderola G, Bailo N, Reinheimer J. Survival of probiotic microflora in yoghurt. Food Res Int. 2000;33(2):97–102. DOI: https://doi.org/10.1016/S0963-9969(00)00011-9