In vitro Antioxidant and free radical scavenging activity of Macrotyloma uniflorum dal from Kumauni region

Abstract

The present study was carried out to evaluate the in vitro antioxidant activities of Methanol
extract of Dolichos biflorus dal ((DME)) commonly edible food from central Himalayans. Methods: This was
achieved by screening of the plant extracts at varying concentrations (20-200μg/ml), using 2,2-diphenyl-1-picryl-
hydrazyl (DPPH) radical scavenging activity, reducing power assay and hydrogen peroxide radical scavenging activity.
Results: Total phenol and flavonoid contents (92.10 ± 8.11 mg/ml GAE per 100 mg plant extract and 139.5 ± 55.09
mg/ml QE equivalent per 100 mg plant extract) were found respectively. Scavenging effect of DME was 4 times greater
than that of the synthetic antioxidant ascorbic acid. Conclusion: Result also suggests a close relations in between total
phenolic content and antioxidant activity, reducing power and radical scavenging effect on DPPH radicals, which proves
Dolichos biflorus has a potential source of useful natural antioxidants.