IDENTIFICATION AND SPECIATION OF DIFFERENT SPECIES OF KLEBSIELLA ISOLATED FROM VARIOUS CLINICAL SAMPLES AND THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL
Main Article Content
Abstract
Klebsiella is a member of the fifth of the eight tribes of the Enterobacteriales family. The second most prevalent organism in the Enterobacteriaceae family is Klebsiella Species. It is a rod-shaped, facultative anaerobic bacillus that ferments lactose and is gram negative. Both hospital-acquired infections & community-acquired infections are known to be frequently caused by them. Additionally, Klebsiella is the second most frequent cause of septicemia and bacteremia at hospital paediatric wards, particularly on intensive care units and premature infants. Klebsiella species are the causative agents of urinary tract infections, pneumonia, abrasion, muscle & bacteremia and account for 3-8% of all nosocomial infections. To identify Klebsiella Species prevalence in different clinical samples along with to evaluate its antibiotic sensitivity method isolated from variety of clinical samples. Over the course of the six- month trial period, 74 samples in total were analyzed. To identify and isolate Klebsiella Species, each of them underwent direct microscopy and culture. In this study 42(57%) Klebsiella Species were isolated in female patients and 32(43%) were from male patients which is identified out of 74 clinical samples. Among the isolates, K. pneumoniae was identified in 65 cases (87.84%) and K. oхytoca in 9 cases (12.16%). Urine and Blood samples where the majority of Klebsiella Species were isolated from specimens. Higher sensitivity was seen with Colistin (100%), Tigecycline (94.59%) and Doxycycline (83.78%) among the 30 urine isolates tested, (83.33%) were sensitive to Fosfomycin by Kirbey-Bauer disc diffusion method. High resistance rates were recorded against: Ceftriaxone (87.84%) and Amoxicillin (79.73%). As an organism with a high prevalence that is growing more common due to antimicrobial agent resistance, Klebsiella Species may pose a risk to health.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
License Statement
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Authors who publish with International Journal of Fundamental and Applied Sciences (IJFAS) retain copyright of their articles and grant the journal the right of first publication.
This license allows others to share, use, and build upon the work—commercially or non-commercially—as long as appropriate credit is given to the original authors and source, and any changes are indicated.
The journal encourages open access and supports the free exchange of knowledge while ensuring proper attribution of original work.
References
Podschun R & Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical microbiology reviews. 1998; 11(4):589-603. DOI: https://doi.org/10.1128/CMR.11.4.589
Kiran Madhusudhan & Bindu D. Antibiotic Susceptibility of Klebsiella pneumoniae Isolated from Various Clinical Samples from a Tertiary Care, Chennai, India. J Res Med Dent Sci. 2022;10(8):264-266.
Archana Bora AB, Aruna Solanki AS, Khatri PK, Parihar RS & Arvind Chandora AC. Detection of carbapenemase in Escherichia and Klebsiella from clinical samples of OPD and IPD patients in tertiary care hospital, Jodhpur, Western Rajasthan, India. Int.J.Curr.Microbiol.App.Sci.2014;3(3): 866-887.
Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and therapeutics. 2015; 40(4):277.
Jondle CN, Gupta K, Mishra BB & Sharma J. Klebsiella pneumoniae infection of murine neutrophils impairs their efferocytic clearance by modulating cell death machinery. PLoS pathogens. 2018; 14(10): e1007338. DOI: https://doi.org/10.1371/journal.ppat.1007338
Reid CB, Steele L, Pasquill K, Parfitt EC, Laupland KB. Occurrence and determinants of Klebsiella species bloodstream infection in the western interior of British Columbia, Canada. BMC Infect Dis. 2019; 19;19(1):1070. DOI: https://doi.org/10.1186/s12879-019-4706-8
Biedenbach DJ, Moet GJ & Jones RN. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997–2002).Diagnostic microbiology and infectious disease. 2004; 50(1):59-69. DOI: https://doi.org/10.1016/j.diagmicrobio.2004.05.003
WHO. Antimicrobial resistance. 2014.
WHO. WHO's first global report on antibiotic resistance reveals serious, worldwide threat to public health. 2014.
Gupta A, Ampofo K, Rubenstein D, & Saiman L. Extended spectrum ẞ lactamase-producing Klebsiella pneumoniae infections: a review of the literature. Journal of perinatology. 2003; 23(6):439-43. DOI: https://doi.org/10.1038/sj.jp.7210973
Wa SW, Dornbusch K. Göransson E. Ransjö U. Kronvall G Characterization of Klebsiella oxytoca septicaemia isolates resistant to aztreonam and cefuroxime. J Antimicrob Chemother 1991; 28; 389-397. DOI: https://doi.org/10.1093/jac/28.3.389
Nathisuwan S, Burgess DS, & Lewis JS. 2nd Extended-spectrum beta-lactamases: epidemiology, detection, and treatment. Pharmacotherapy. 2001; 21; 920-928. DOI: https://doi.org/10.1592/phco.21.11.920.34529
Shilpa K, Thomas R & Ramyashree A. Isolation and antimicrobial sensitivity pattern of Klebsiella pneumoniae from sputum samples in a tertiary care hospital. Int J Curr Microbiol App Sci. 2016;5(12):1.
Tufanova OS, Kasimova AR, Astakhov DI, Rukina AN & Bozhkova SA. Factors affecting the course and prognosis of implant-associated infection caused by Klebsiella spp. Traumatology and Orthopedics of Russia. 2024; 430(2):40-53. DOI: https://doi.org/10.17816/2311-2905-16719
Sudhaharan S, Kanne P, Vemu L, Chavali P, Desmukha SR & Nagari B. Bacteriological profile of intra-abdominal infections in a tertiary care hospital. Iran J Microbiol. 2018; 10(4):208-214.
Fareed M. Prevalence and antimicrobial susceptibility of Klebsiella species in clinical specimens of both indoor and outdoor patients of tertiary care hospital, Rawalpindi. Int J Med Sci. 2024; 12(3):45-50.
Mwangi W & Kibuchi J. Antibiotic resistance patterns of Klebsiella pneumoniae isolated from clinical specimens in a tertiary hospital in Kenya. Pan Afr Med J. 2019; 32:140.
Dhurve R. Prevalence and antimicrobial susceptibility pattern of Klebsiella species isolated from clinical samples in a tertiary care hospital. Int J Res Med Sci. 2018;6(2):566-70.