Determination of genetic fidelity of Micropropagated plants of Zingiber officinale cv-Majhauley of Sikkim Himalaya using RAPD markers

Abstract

The present investigation was carried out for the determination of genetic conformity of micropropagated plants of Zingiber
officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) technique.Total fifteen different arbitrary
decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 18 months old in
vitro cultured plantlets. Total 38 amplified reproducible bands were produced from 10 primers with an average of 3.8 per
RAPD primer, amplified bands were ranging from 150-950 base pair, the highest seven number of bands found in primer
CLT53 (50-875 bp) and lowest one band in CLT 112 (575 bp) and CLT 192 (650 bp) primers. All the primers were found to be
monomorphic and no genetic variation was detected within the micropropagated plants. Present protocol may be used for
testing of ginger propagules to ensure mass production of disease free planting materials